{"id":1128,"date":"2019-04-22T07:30:49","date_gmt":"2019-04-22T07:30:49","guid":{"rendered":"https:\/\/albayan.edu.iq\/en\/?p=1128"},"modified":"2019-04-22T07:30:49","modified_gmt":"2019-04-22T07:30:49","slug":"evaluation-of-miltefosine-activity-against-viability-and-dna-integrity-of-leishmania-donovani-promastigotes-in-vitro","status":"publish","type":"post","link":"https:\/\/albayan.edu.iq\/en\/2019\/04\/22\/1128\/","title":{"rendered":"EVALUATION OF MILTEFOSINE ACTIVITY AGAINST VIABILITY AND  DNA INTEGRITY OF LEISHMANIA DONOVANI PROMASTIGOTES IN VITRO"},"content":{"rendered":"<p style=\"text-align: justify;\"><strong>Braa A. Alshakir and Khawla H. Zghair<\/strong><\/p>\n<p style=\"text-align: justify;\"><strong>ABSTRACT<\/strong><\/p>\n<p style=\"text-align: justify;\">Visceral leishmaniasis is the most severe disease from all leishmaniasis forms and can be fatal without treatment. Miltefosine was the first oral drug approved in several countries for treatment of visceral and cutaneous leishmaniasis. Regional variations in susceptibility of <em>Leishmania donovani <\/em>clinical isolates have been recorded to sodium stibogluconate but not other antileishmanial drugs. This study investigates the viability of <em>Leishmania<\/em><em>donovani <\/em>promastigotes using the colorimetric MTT assay and DNA integrity in vitro after exposed to different concentrations of miltefosine in comparison to untreated parasites. The results were expressed as inhibitory concentration IC50. The IC50 of miltefosine drug after 24, 48 and 72 hr was 13.32 \u03bcM, 11.73\u03bcM and 10.63\u03bcM against <em>L. donovani <\/em>promastigotes respectively. Miltefosine induce apoptosis like death in <em>L. donovani<\/em>promastigotes, Degradation of DNA was revealed at 11.04, 12.26, 14.72 and 17.17\u03bcM after 24 hr, and the clearly degradation was noticed for these concentrations of miltefosine drug after 48 hr ,while the highest concentration of miltefosine (19.62\u03bcM) caused completely lysis of DNA after 24hr. Miltefosine in lower concentrations affect <em>L.donovani <\/em>viability and induce apoptosis like death, thus it might be a promising approach for developing new oral anti- visceral Leishmanial drug.<\/p>\n<p style=\"text-align: justify;\"><strong>For full article click the link below:<\/strong><\/p>\n<p style=\"text-align: justify;\"><a href=\"https:\/\/www.researchgate.net\/publication\/324993944_EVALUATION_OF_MILTEFOSINE_ACTIVITY_AGAINST_VIABILITY_AND_DNA_INTEGRITY_OF_LEISHMANIA_DONOVANI_PROMASTIGOTES_IN_VITRO\" target=\"_blank\" rel=\"noopener noreferrer\">https:\/\/www.researchgate.net\/publication\/324993944<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Braa A. Alshakir and Khawla H. Zghair ABSTRACT Visceral leishmaniasis is the most severe disease from all leishmaniasis forms and can be fatal without treatment. Miltefosine was the first oral drug approved in several countries for treatment of visceral and cutaneous leishmaniasis. Regional variations in susceptibility of Leishmania donovani clinical isolates have been recorded to sodium stibogluconate but not other antileishmanial drugs. This study investigates the viability of Leishmaniadonovani promastigotes using the colorimetric MTT assay and DNA integrity in vitro after exposed to different concentrations of miltefosine in comparison to untreated parasites. The results were expressed as inhibitory concentration IC50. The <a href=\"https:\/\/albayan.edu.iq\/en\/2019\/04\/22\/1128\/\"> [&#8230;]<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[26],"tags":[27],"class_list":["post-1128","post","type-post","status-publish","format-standard","hentry","category-staff-researches","tag-staff-researches"],"jetpack_featured_media_url":"","jetpack_shortlink":"https:\/\/wp.me\/paQN8v-ic","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/albayan.edu.iq\/en\/wp-json\/wp\/v2\/posts\/1128","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/albayan.edu.iq\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/albayan.edu.iq\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/albayan.edu.iq\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/albayan.edu.iq\/en\/wp-json\/wp\/v2\/comments?post=1128"}],"version-history":[{"count":1,"href":"https:\/\/albayan.edu.iq\/en\/wp-json\/wp\/v2\/posts\/1128\/revisions"}],"predecessor-version":[{"id":1130,"href":"https:\/\/albayan.edu.iq\/en\/wp-json\/wp\/v2\/posts\/1128\/revisions\/1130"}],"wp:attachment":[{"href":"https:\/\/albayan.edu.iq\/en\/wp-json\/wp\/v2\/media?parent=1128"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/albayan.edu.iq\/en\/wp-json\/wp\/v2\/categories?post=1128"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/albayan.edu.iq\/en\/wp-json\/wp\/v2\/tags?post=1128"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}